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KMID : 0620919970290040203
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Experimental & Molecular Medicine
1997 Volume.29 No. 4 p.203 ~ p.208
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Stimulation of c-myc protooncogene expression by transforming growth factor α in human ovarian cancer cells
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Hae Young Park/Jeong Youn Choi
Young Sook Hong/Hae Young Park
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Abstract
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To investigate whether transforming growth factor α (TGF α) treatment of human ovarian cancer cells was associated with the induction of c-myc protooncogene, the expression of this gene in NIH:OVCAR-3 cells was examined. TGF α induced increase in c-myc mRNA level, with a peak after 1 h of treatment; this stimulation was dose-dependent, with an optimal concentration of 5 ng/ml TGF α. Its primary action is probably at the transcription level since the half-life of c-myc mRNA measured in the presence of actinomycin D was not modified by TGF α treatment. In addition, TGF α stimulation of c-myc mRNA did not require protein synthesis since it was not suppressed by cycloheximide treatment. Antisense phosphorothioate oligonucleotide to c-myc specifically inhibited the TGF α-stimulated c-Myc protein expression and growth of NIH:OVCAR-3 cells. Our results indicate that induction of c-myc expression by TGF α plays an important role in the growth of NIH:OVCAR-3 cells.
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KEYWORD
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c-myc, ovarian cancer cells, TGFα,
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